Sequencing and BLAST
Sequencing:
Four samples were chosen from the 8 samples that were mini-preped based off the concentrations of DNA for each sample. The samples with the highest concentrations were chosen. Each sample was diluted to 50ng/µL and 10µL of each sample was sent to a company called Genewiz for DNA sequencing.
BLAST:
Once the sequences were sent back to us, the sequences of DNA were run through an online database to identify the bacterial and fungal species.
Four samples were chosen from the 8 samples that were mini-preped based off the concentrations of DNA for each sample. The samples with the highest concentrations were chosen. Each sample was diluted to 50ng/µL and 10µL of each sample was sent to a company called Genewiz for DNA sequencing.
BLAST:
Once the sequences were sent back to us, the sequences of DNA were run through an online database to identify the bacterial and fungal species.