To identify bacterial feces, DNA was first extracted from samples of bacterial species. Then the extracted DNA was run through a Polymerase Chain Reaction. A polymerase chain reaction amplifies specific regions of DNA. In this case, we targeted the 16S and 18S rDNA regions. The amplified DNA was then run through a process called gel electrophoresis to make sure the correct gene fragments were amplified. The PCR samples were then inserted into a plasmid through a process a called ligation. The recombinant plasmid was then taken up by E. coli through a process called transformation. The transformed bacteria grew in colonies on plates containing ampicillin. Major colonies were chosen from each plate and the DNA was extracted from each plate. The extracted DNA was then sent to a company called Genewiz and where each sample was sequenced. The sequences were sent back and ran it through a Basic Local Alignment Search Tool, or BLAST, and identified three bacterial species and one fungal species.